Our research is focused on developing a comprehensive understanding of metabolic processes, particularly physiological ‘state changes’ that occur as a result of environmental perturbations. A major challenge has been the lack of tools to resolve these processes both in terms of network topology and spatial localization. Hence, we have made major progress pioneering methods for gas phase ion generation and applying mass spectrometry for studying metabolites in complex biological systems. Central to these efforts are the development of experimental and computational approaches to study these processes in space and time using activity profiling, stable isotope tracing, and mass spectrometry imaging.
We are interested in dynamic metabolic responses of cells to environmental perturbations ranging from shifts in nutrient availability to environmental stresses. Central to these efforts are the development of experimental and computational approaches to study these processes using mass spectrometry based metabolite and activity profiling, stable isotope tracing, and mass spectrometry imaging. Together these allow us to comprehensively characterize metabolic activities, dynamics and localization within complex cellular systems to predict responses and design interventions.